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ezrin specific sirna (Santa Cruz Biotechnology)

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LPS induced <t>ezrin</t> phosphorylation in a concentration- and time-dependent manner. A549 and HPAEpiC cells were treated with LPS for 3 h at concentrations of 0 µg/mL, 0.1 µg/mL, 1 µg/mL, and 10 µg/mL, respectively. Phosphorylated ezrin protein level was evaluated by western blotting ( a , b ) and flow cytometry ( c , d ). A549 and HPAEpiC cells were treated with LPS (1 µg/mL) for 0 h, 0.5 h, 1 h, 3 h, 6 h and 12 h, respectively. Phosphorylated ezrin protein level was evaluated by western blotting ( e , f ) and flow cytometry ( g , h ). Data are expressed as means ± SD of triplicate samples. * p < 0.05 versus 0 µg/mL group

Ezrin Specific Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ezrin+specific+sirna/Ezrin+siRNA/pmc09097379-60-16-26
Average 93 stars, based on 20 article reviews

ezrin specific sirna - by Bioz Stars, 2026-09

93/100 stars

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1) Product Images from "The ROCK-ezrin signaling pathway mediates LPS-induced cytokine production in pulmonary alveolar epithelial cells"

Article Title: The ROCK-ezrin signaling pathway mediates LPS-induced cytokine production in pulmonary alveolar epithelial cells

Journal: Cell Communication and Signaling : CCS

doi: 10.1186/s12964-022-00879-3

LPS induced ezrin phosphorylation in a concentration- and time-dependent manner. A549 and HPAEpiC cells were treated with LPS for 3 h at concentrations of 0 µg/mL, 0.1 µg/mL, 1 µg/mL, and 10 µg/mL, respectively. Phosphorylated ezrin protein level was evaluated by western blotting ( a , b ) and flow cytometry ( c , d ). A549 and HPAEpiC cells were treated with LPS (1 µg/mL) for 0 h, 0.5 h, 1 h, 3 h, 6 h and 12 h, respectively. Phosphorylated ezrin protein level was evaluated by western blotting ( e , f ) and flow cytometry ( g , h ). Data are expressed as means ± SD of triplicate samples. * p < 0.05 versus 0 µg/mL group

Figure Legend Snippet: LPS induced ezrin phosphorylation in a concentration- and time-dependent manner. A549 and HPAEpiC cells were treated with LPS for 3 h at concentrations of 0 µg/mL, 0.1 µg/mL, 1 µg/mL, and 10 µg/mL, respectively. Phosphorylated ezrin protein level was evaluated by western blotting ( a , b ) and flow cytometry ( c , d ). A549 and HPAEpiC cells were treated with LPS (1 µg/mL) for 0 h, 0.5 h, 1 h, 3 h, 6 h and 12 h, respectively. Phosphorylated ezrin protein level was evaluated by western blotting ( e , f ) and flow cytometry ( g , h ). Data are expressed as means ± SD of triplicate samples. * p < 0.05 versus 0 µg/mL group

Techniques Used: Phospho-proteomics, Concentration Assay, Western Blot, Flow Cytometry

ROCK mediated LPS-induced ezrin phosphorylation and translocation. A549 and HPAEpiC cells were challenged with LPS (1 µg/mL) in the presence or absence of ROCK siRNA or Y-27632. Total ezrin and p-ezrin protein levels were detected by western blotting using β-actin as an internal reference ( a , b ). Data are expressed as means ± SD of triplicate samples. * p < 0.05 versus control, # p < 0.05 versus LPS. The intracellular localization of p-ezrin in resting and LPS-activated cells was investigated by immunofluorescence. P-ezrin was stained with Alexa Fluor 488-conjugated IgG (green), F-actin was stained with Rhodamine-phalloidin (yellow), and nuclei were stained with DAPI (blue) ( c , d ). Qualitative analysis was performed by confocal microscopy. Scale bar, 10 μm. All experiments were performed in three independent experiments

Figure Legend Snippet: ROCK mediated LPS-induced ezrin phosphorylation and translocation. A549 and HPAEpiC cells were challenged with LPS (1 µg/mL) in the presence or absence of ROCK siRNA or Y-27632. Total ezrin and p-ezrin protein levels were detected by western blotting using β-actin as an internal reference ( a , b ). Data are expressed as means ± SD of triplicate samples. * p < 0.05 versus control, # p < 0.05 versus LPS. The intracellular localization of p-ezrin in resting and LPS-activated cells was investigated by immunofluorescence. P-ezrin was stained with Alexa Fluor 488-conjugated IgG (green), F-actin was stained with Rhodamine-phalloidin (yellow), and nuclei were stained with DAPI (blue) ( c , d ). Qualitative analysis was performed by confocal microscopy. Scale bar, 10 μm. All experiments were performed in three independent experiments

Techniques Used: Phospho-proteomics, Translocation Assay, Western Blot, Control, Immunofluorescence, Staining, Confocal Microscopy

The association between ezrin, MyD88/IRAK1, and Syk. A549 cells were challenged with LPS (1 µg/mL) in the presence or absence of ROCK siRNA or ezrin siRNA. Lysates isolated from A549 cells exposed to LPS (+) or without LPS (−) were immunoprecipitated in the presence of anti-ezrin or control IgG antibody, followed by western blotting with anti-Syk, anti-MyD88, or anti-IRAK1 antibodies, respectively. IP: immunoprecipitation, IB: immunoblotting

Figure Legend Snippet: The association between ezrin, MyD88/IRAK1, and Syk. A549 cells were challenged with LPS (1 µg/mL) in the presence or absence of ROCK siRNA or ezrin siRNA. Lysates isolated from A549 cells exposed to LPS (+) or without LPS (−) were immunoprecipitated in the presence of anti-ezrin or control IgG antibody, followed by western blotting with anti-Syk, anti-MyD88, or anti-IRAK1 antibodies, respectively. IP: immunoprecipitation, IB: immunoblotting

Techniques Used: Isolation, Immunoprecipitation, Control, Western Blot

The suppression of ezrin inhibited LPS-induced activation of p38 and NF-κB. A549 cells were transfected with ezrin siRNA or control siRNA. The expression of ezrin mRNA was detected by qRT-PCR ( a ) and the protein level of ezrin was measured by western blotting ( b ). The effect of ezrin siRNA transfection on IKK, IκBα, and MAPKs activation was detected by western blotting ( c – h ), and NF-κB activation was determined by EMSA (i). Data are expressed as mean ± SD of triplicate samples. * p < 0.05 versus LPS(-) control siRNA group, # p < 0.05 versus LPS(+) control siRNA group

Figure Legend Snippet: The suppression of ezrin inhibited LPS-induced activation of p38 and NF-κB. A549 cells were transfected with ezrin siRNA or control siRNA. The expression of ezrin mRNA was detected by qRT-PCR ( a ) and the protein level of ezrin was measured by western blotting ( b ). The effect of ezrin siRNA transfection on IKK, IκBα, and MAPKs activation was detected by western blotting ( c – h ), and NF-κB activation was determined by EMSA (i). Data are expressed as mean ± SD of triplicate samples. * p < 0.05 versus LPS(-) control siRNA group, # p < 0.05 versus LPS(+) control siRNA group

Techniques Used: Activation Assay, Transfection, Control, Expressing, Quantitative RT-PCR, Western Blot

The suppression of ezrin inhibited the LPS-induced production of cytokines. A549 cells were pre-treated with Y-27632 and subjected to LPS. The release of TNF-α, IL-1β, and HMGB1 into the supernatants was then measured by ELISA ( a – c ). The expression level of HMGB1 in cell lysates was measured by western blotting ( d ). Cell viability was examined by CCK-8 assay ( e ). A549 cells were transfected with ezrin-specific siRNA; TNF-α, and IL-1β expression was measured by ELISA ( f – h ), and the cellular levels of HMGB1 was measured by western blotting ( i ). Data are expressed as mean ± SD of triplicate samples. * p < 0.05 versus control group, # p < 0.05 versus LPS group

Figure Legend Snippet: The suppression of ezrin inhibited the LPS-induced production of cytokines. A549 cells were pre-treated with Y-27632 and subjected to LPS. The release of TNF-α, IL-1β, and HMGB1 into the supernatants was then measured by ELISA ( a – c ). The expression level of HMGB1 in cell lysates was measured by western blotting ( d ). Cell viability was examined by CCK-8 assay ( e ). A549 cells were transfected with ezrin-specific siRNA; TNF-α, and IL-1β expression was measured by ELISA ( f – h ), and the cellular levels of HMGB1 was measured by western blotting ( i ). Data are expressed as mean ± SD of triplicate samples. * p < 0.05 versus control group, # p < 0.05 versus LPS group

Techniques Used: Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, CCK-8 Assay, Transfection, Control

Figure Legend Snippet: A schematic summary of the ROCK-ezrin pathway in response to LPSs

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Article Title: The ROCK-ezrin signaling pathway mediates LPS-induced cytokine production in pulmonary alveolar epithelial cells
Article Snippet: Samples were washed three times with TBST prior to confocal microscopy (Olympus FV1000, Olympus, Tokyo, Japan). .. For transient knockdown experiments, cells were transfected with ROCK1-specific siRNA (ROCK1 siRNA) or control siRNA, and ezrin specific siRNA (ezrin siRNA) or control siRNA (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA), respectively. ..

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Journal: Cell Communication and Signaling : CCS

Article Title: The ROCK-ezrin signaling pathway mediates LPS-induced cytokine production in pulmonary alveolar epithelial cells

doi: 10.1186/s12964-022-00879-3

Figure Lengend Snippet: LPS induced ezrin phosphorylation in a concentration- and time-dependent manner. A549 and HPAEpiC cells were treated with LPS for 3 h at concentrations of 0 µg/mL, 0.1 µg/mL, 1 µg/mL, and 10 µg/mL, respectively. Phosphorylated ezrin protein level was evaluated by western blotting ( a , b ) and flow cytometry ( c , d ). A549 and HPAEpiC cells were treated with LPS (1 µg/mL) for 0 h, 0.5 h, 1 h, 3 h, 6 h and 12 h, respectively. Phosphorylated ezrin protein level was evaluated by western blotting ( e , f ) and flow cytometry ( g , h ). Data are expressed as means ± SD of triplicate samples. * p < 0.05 versus 0 µg/mL group

Article Snippet: For transient knockdown experiments, cells were transfected with ROCK1-specific siRNA (ROCK1 siRNA) or control siRNA, and ezrin specific siRNA (ezrin siRNA) or control siRNA (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA), respectively.

Techniques: Phospho-proteomics, Concentration Assay, Western Blot, Flow Cytometry

Journal: Cell Communication and Signaling : CCS

Article Title: The ROCK-ezrin signaling pathway mediates LPS-induced cytokine production in pulmonary alveolar epithelial cells

doi: 10.1186/s12964-022-00879-3

Figure Lengend Snippet: ROCK mediated LPS-induced ezrin phosphorylation and translocation. A549 and HPAEpiC cells were challenged with LPS (1 µg/mL) in the presence or absence of ROCK siRNA or Y-27632. Total ezrin and p-ezrin protein levels were detected by western blotting using β-actin as an internal reference ( a , b ). Data are expressed as means ± SD of triplicate samples. * p < 0.05 versus control, # p < 0.05 versus LPS. The intracellular localization of p-ezrin in resting and LPS-activated cells was investigated by immunofluorescence. P-ezrin was stained with Alexa Fluor 488-conjugated IgG (green), F-actin was stained with Rhodamine-phalloidin (yellow), and nuclei were stained with DAPI (blue) ( c , d ). Qualitative analysis was performed by confocal microscopy. Scale bar, 10 μm. All experiments were performed in three independent experiments

Techniques: Phospho-proteomics, Translocation Assay, Western Blot, Control, Immunofluorescence, Staining, Confocal Microscopy

Journal: Cell Communication and Signaling : CCS

Article Title: The ROCK-ezrin signaling pathway mediates LPS-induced cytokine production in pulmonary alveolar epithelial cells

doi: 10.1186/s12964-022-00879-3

Figure Lengend Snippet: The association between ezrin, MyD88/IRAK1, and Syk. A549 cells were challenged with LPS (1 µg/mL) in the presence or absence of ROCK siRNA or ezrin siRNA. Lysates isolated from A549 cells exposed to LPS (+) or without LPS (−) were immunoprecipitated in the presence of anti-ezrin or control IgG antibody, followed by western blotting with anti-Syk, anti-MyD88, or anti-IRAK1 antibodies, respectively. IP: immunoprecipitation, IB: immunoblotting

Techniques: Isolation, Immunoprecipitation, Control, Western Blot

Journal: Cell Communication and Signaling : CCS

Article Title: The ROCK-ezrin signaling pathway mediates LPS-induced cytokine production in pulmonary alveolar epithelial cells

doi: 10.1186/s12964-022-00879-3

Figure Lengend Snippet: The suppression of ezrin inhibited LPS-induced activation of p38 and NF-κB. A549 cells were transfected with ezrin siRNA or control siRNA. The expression of ezrin mRNA was detected by qRT-PCR ( a ) and the protein level of ezrin was measured by western blotting ( b ). The effect of ezrin siRNA transfection on IKK, IκBα, and MAPKs activation was detected by western blotting ( c – h ), and NF-κB activation was determined by EMSA (i). Data are expressed as mean ± SD of triplicate samples. * p < 0.05 versus LPS(-) control siRNA group, # p < 0.05 versus LPS(+) control siRNA group

Techniques: Activation Assay, Transfection, Control, Expressing, Quantitative RT-PCR, Western Blot

Journal: Cell Communication and Signaling : CCS

Article Title: The ROCK-ezrin signaling pathway mediates LPS-induced cytokine production in pulmonary alveolar epithelial cells

doi: 10.1186/s12964-022-00879-3

Figure Lengend Snippet: The suppression of ezrin inhibited the LPS-induced production of cytokines. A549 cells were pre-treated with Y-27632 and subjected to LPS. The release of TNF-α, IL-1β, and HMGB1 into the supernatants was then measured by ELISA ( a – c ). The expression level of HMGB1 in cell lysates was measured by western blotting ( d ). Cell viability was examined by CCK-8 assay ( e ). A549 cells were transfected with ezrin-specific siRNA; TNF-α, and IL-1β expression was measured by ELISA ( f – h ), and the cellular levels of HMGB1 was measured by western blotting ( i ). Data are expressed as mean ± SD of triplicate samples. * p < 0.05 versus control group, # p < 0.05 versus LPS group

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, CCK-8 Assay, Transfection, Control

Journal: Cell Communication and Signaling : CCS

Article Title: The ROCK-ezrin signaling pathway mediates LPS-induced cytokine production in pulmonary alveolar epithelial cells

doi: 10.1186/s12964-022-00879-3

Figure Lengend Snippet: A schematic summary of the ROCK-ezrin pathway in response to LPSs

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